Introduction

Entomopathogenic nematodes are biological control agents of arthropod pests that can be multiplied by in vivo and in vitro methods. They are effective when well marched with host arthropods but the major drawback to their wide use is their availability on demand. The in vivo method is appropriate for a cottage industry and the in vitro method for massive production.

Abstract

Entomopathogenic nematodes are biological control agents of arthropod pests that can be multiplied by in vivo and in vitro methods. They are effective when well marched with host arthropods but the major drawback to their wide use is their availability on demand. The in vivo method is appropriate for a cottage industry and the in vitro method for massive production.

Methodology

Three hundred Petri dishes were lined with Whatman filter paper and the nematodes Steinernema karii, Heterorhabditis indica and Steinernema yirgalemense applied at the rate of 100 ij per dish (9 x 3.5) in fifty dishes for each nematode species and at 200ij/dish to another batch of fifty for each nematode species. The nematodes were applied in 1ml distilled water and given 30 minutes to distribute on the filter.

Summary

Costa, J.R., Dias, R.J.P., and Morenze, M.J.F. (2007). Determining the adaptation potential of multiplication of a Heterorhabditis sp and Steinernema carpocapsae (Rhabditidae::Heterorhabdtidae and Steinernematidae) in larvae of Alphitobius diaperinus (Coleoptera: Terebrionidae) and Galleria mellonella (Lepidoptera

Pyralidae). Journal of Parasitology. 102,, 139-144.

Ehlers, R.U., Linau, S., Krasomil O., Osterfield ,K.H. 1998. Liquid culture of Entomopathogenic nematode –bacterium complex Heterorhabditis megidis /Photorhabdus luminescence. Bio Control. 43(1), 77-88.

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African Dryland Institute for Sustainability , University of Nairobi